Macromolecular Crystallography Protocols

Macromolecular Crystallography Protocols

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Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1, Preparation and Crystallization of Macromolecules and Volume 2, Structure Determination, explore recent advances that have accelerated the pace of structural determination and made crystallography accessible to a broader range of investigators. Volume 1 is composed of detailed protocols for the preparation and optimization of crystals, including tips from the experts on the best methods for inducing proteins to adopt their crystalline form. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization, as well as computational techniques for data collection, phasing, and refinement. The volume concludes with a detailed and insightful survey of available crystallographic software. These volumes will be an indispensable reference for obtaining macromolecular crystals and determining their three-dimensional structure.His6-TEV protease is active in all buffers compatible with IMAC. ... 24. Although the passenger protein is typically free of contaminants after the second IMAC step , it may still exist as a mixture of oligomeric forms including some high molecular anbsp;...

Title:Macromolecular Crystallography Protocols
Author: Sylvie Doublié
Publisher:Springer Science & Business Media - 2007

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